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topflash
Topflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m50+super+8x+topflash+plasmid/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm41906239-113-12-13
Average 96 stars, based on 573 article reviews
Topflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m50+super+8x+topflash+plasmid/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm41906239-113-12-13
Average 96 stars, based on 573 article reviews
topflash - by Bioz Stars,
2026-09
96/100 stars
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Luciferase:Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), Reporter Assay:Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), Mutagenesis:Article Title: Article Snippet: A total of 2 μg of DKK1 promoter and 0.5 μg of pRL (Promega, E2261) plasmids were transfected into SH-SY5Y cells (7.5×105) using FuGENE HD Transfection Reagent (Promega) in a 6-well culture plate. .. For the TCF/LEF luciferase reporter assay (TOPFlash), Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 10 6 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor. Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 106 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Concentration Assay:Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 10 6 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor. Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 106 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Plasmid Preparation:Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 10 6 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: Article Title: Elucidation of molecular basis of osteolytic bone lesions in advanced multiple myeloma Article Snippet: .. The cells were transfected with pcDNA3.1-empty vector or pcDNA3.1-DKK1, Article Title: Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta. Article Snippet: UN CO RR EC TE D MA NU SC RI PT Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta Zhijia Tan1,2,3†*, Peikai Chen1,2,4,6†, Jianan Zhang3,5†, Hiu Tung Shek 1,2, Zeluan Li1,2, Xinlin Zhou1, Yapeng Zhou1,2, Shijie Yin1,2, Lina Dong1,2, Lin Feng1,2, Janus Siu Him Wong1,2,3, Bo Gao1,2,5*, Michael Kai Tsun To1,2,3* 1Department of Orthopaedics and Traumatology, The University of Hong KongShenzhen Hospital, Shenzhen, China; 2Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China; 3Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong; 4School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong; 5School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong; 6The AI and Big data lab, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.. Address correspondence to: Zhijia Tan, Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.. Email: tanzj@hku-szh.org Bo Gao, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong. Article Title: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor. Article Snippet: .. HEK293T cells were plated in 6-well plates at a concentration of 1 × 106 cells per well and co-transfected with 500 ng of wt or mutant LRP6-GFP or GFP empty vector plasmids, 250 ng of Article Title: Syndecan-2 positively regulates Wnt/β-catenin signaling in breast cancer cells. Article Snippet: Syndecans are a family of four-member transmembrane heparan sulfate proteoglycans that bind to various extracellular biomolecules, such as Wnt ligands, via their heparan sulfate chains, thereby controlling a variety of cellular processes.. When dysregulated, syndecans can affect tumorigenesis and cancer progression by modulating key signaling pathways involved in the regulation of biological functions.. Aberrant activation of Wnt/β-catenin signaling is a hallmark of many human tumors, including breast cancer. Article Title: Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta Article Snippet: .. Another batch of HEK293T cells was prepared for transfection with Transfection:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: Article Title: Elucidation of molecular basis of osteolytic bone lesions in advanced multiple myeloma Article Snippet: .. The cells were transfected with pcDNA3.1-empty vector or pcDNA3.1-DKK1, Article Title: Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta. Article Snippet: UN CO RR EC TE D MA NU SC RI PT Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta Zhijia Tan1,2,3†*, Peikai Chen1,2,4,6†, Jianan Zhang3,5†, Hiu Tung Shek 1,2, Zeluan Li1,2, Xinlin Zhou1, Yapeng Zhou1,2, Shijie Yin1,2, Lina Dong1,2, Lin Feng1,2, Janus Siu Him Wong1,2,3, Bo Gao1,2,5*, Michael Kai Tsun To1,2,3* 1Department of Orthopaedics and Traumatology, The University of Hong KongShenzhen Hospital, Shenzhen, China; 2Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China; 3Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong; 4School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong; 5School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong; 6The AI and Big data lab, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.. Address correspondence to: Zhijia Tan, Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.. Email: tanzj@hku-szh.org Bo Gao, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong. Article Title: Syndecan-2 positively regulates Wnt/β-catenin signaling in breast cancer cells. Article Snippet: Syndecans are a family of four-member transmembrane heparan sulfate proteoglycans that bind to various extracellular biomolecules, such as Wnt ligands, via their heparan sulfate chains, thereby controlling a variety of cellular processes.. When dysregulated, syndecans can affect tumorigenesis and cancer progression by modulating key signaling pathways involved in the regulation of biological functions.. Aberrant activation of Wnt/β-catenin signaling is a hallmark of many human tumors, including breast cancer. Article Title: Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta Article Snippet: .. Another batch of HEK293T cells was prepared for transfection with Control:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: Article Title: Elucidation of molecular basis of osteolytic bone lesions in advanced multiple myeloma Article Snippet: .. The cells were transfected with pcDNA3.1-empty vector or pcDNA3.1-DKK1, Construct:Article Title: Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo Article Snippet: .. On the day of transfection, each well received the following plasmids: |
